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plko 1 puro shctnnb1  (Addgene inc)


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    Structured Review

    Addgene inc plko 1 puro shctnnb1
    Plko 1 Puro Shctnnb1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+puro+shctnnb1/pLKO%2E1+puro+shRNA+beta-catenin+(Plasmid+%2318803)/pmc07532415-69-3-12
    Average 93 stars, based on 34 article reviews
    plko 1 puro shctnnb1 - by Bioz Stars, 2026-10
    93/100 stars

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    Plasmid Preparation:

    Article Title: Essentiality of CTNNB1 in Malignant Transformation of Human Embryonic Stem Cells under Long-Term Suboptimal Conditions
    Article Snippet: .. CTNNB1 shRNAs plasmid, pLKO.1-puro-shCTNNB1 (pLKO.1 puro shRNA beta-catenin, Plasmid #18803) were from Addgene, with empty vector (pLKO.1 puro, Plasmid, #8453) as control. .. Viral particles were packaged in virus packaging cell line 293FT and collected according to the manufacturer's instructions, then added to mTeSRTM1 medium (StemCell Technologies, Canada) with 8 ng/ml polybrene and incubated overnight; the second infection was performed on the next day.

    shRNA:

    Article Title: Essentiality of CTNNB1 in Malignant Transformation of Human Embryonic Stem Cells under Long-Term Suboptimal Conditions
    Article Snippet: .. CTNNB1 shRNAs plasmid, pLKO.1-puro-shCTNNB1 (pLKO.1 puro shRNA beta-catenin, Plasmid #18803) were from Addgene, with empty vector (pLKO.1 puro, Plasmid, #8453) as control. .. Viral particles were packaged in virus packaging cell line 293FT and collected according to the manufacturer's instructions, then added to mTeSRTM1 medium (StemCell Technologies, Canada) with 8 ng/ml polybrene and incubated overnight; the second infection was performed on the next day.

    Control:

    Article Title: Essentiality of CTNNB1 in Malignant Transformation of Human Embryonic Stem Cells under Long-Term Suboptimal Conditions
    Article Snippet: .. CTNNB1 shRNAs plasmid, pLKO.1-puro-shCTNNB1 (pLKO.1 puro shRNA beta-catenin, Plasmid #18803) were from Addgene, with empty vector (pLKO.1 puro, Plasmid, #8453) as control. .. Viral particles were packaged in virus packaging cell line 293FT and collected according to the manufacturer's instructions, then added to mTeSRTM1 medium (StemCell Technologies, Canada) with 8 ng/ml polybrene and incubated overnight; the second infection was performed on the next day.



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